Comparison of Cytotoxic Activity of Anticancer Drugs against Various Human Tumor Cell Lines Using In Vitro Cell-Based Approach
نویسندگان
چکیده
Chemotherapy is the main treatment modality for certain types of cancer. It is important to monitor and ensure that these chemotherapeutic drugs are potent and effective prior to patient administration. The objective of the study is to evaluate the cytotoxic activity and potency of selected commercially available generic anticancer drugs in comparison with originator using various human cancer cell lines in an in vitro cell-based assay. Half-maximal inhibitory concentration (IC50) of the different chemotherapeutic agents was obtained from an experimentally derived dose-response curve. Relative potency of the drugs was estimated according to Parallel Line assay. This study demonstrated that the selected generic oncology products tested had similar efficacy compared with the originator. Both products showed comparable results as shown both in vitro cytotoxicity assay and statistical analysis. In vitro cell-based cytotoxicity assay promises to be a useful, reliable and rapid method for demonstrating chemotherapeutic drug activity.
منابع مشابه
Novel Approach Synthesis, Molecular Docking and Cytotoxic Activity Evaluation of N-phenyl-2,2-dichloroacetamide Derivatives as Anticancer Agents
Dichloroacetate (DCA) as a small, cheap and available anticancer agent, is a pyruvate mimetic compound that stimulates the activity of pyruvate dehydrogenase (PDH) enzyme through inhibition of pyruvate dehydrogenase kinases (PDHK1-4). DCA turns on programed cell death (apoptosis) which suppressed in tumor cells and therefore inhibits tumor growth. DCA also interferes with the glucose uses of ca...
متن کاملIn Vitro Cytotoxic Activity of the Essential Oil Extracted from Artemisia Absinthium
Background: Essential oils are found to have multiple active components which can show in vitro cytotoxic action against various cancerous cell lines. This study reports the in vitro cytotoxic effects of the essential oil from Artemisia absinthium L. (Asteraceae) growing wild in Iran. Methods: Water-distilled essential oil of A. absinthium collected from Ardabil, north-western Iran, was examin...
متن کاملSynthesis and Evaluation of the Cytotoxicity of a Series of 1,3,4-Thiadiazole Based Compounds as Anticancer Agents
Objective(s): Nowadays, cancer is an important public health problem in all countries. Limitations of current chemotherapy for neoplastic diseases such as severe adverse reactions and tumor resistance to the chemotherapeutic drugs have been led to a temptation for focusing on the discovery and development of new compounds with potential anticancer activity. Materials and Metho...
متن کاملDiterpenoids from Roots of Salvia lachnocalyx; In-silico and In-vitro Toxicity against Human Cancer Cell Lines
Further investigations on phytochemical constituents of dichloromethane extract fromroots of Salvia lachnocalyx (S. lachnocalyx) led to the isolation and identification of eightknown diterpenoids from this plant for the first time. The chemical structures of the purifiedcompounds were elucidated using spectroscopic analyses including EI-MS, 1H and 13C NMR andby compariso...
متن کاملDiterpenoids from Roots of Salvia lachnocalyx; In-silico and In-vitro Toxicity against Human Cancer Cell Lines
Further investigations on phytochemical constituents of dichloromethane extract fromroots of Salvia lachnocalyx (S. lachnocalyx) led to the isolation and identification of eightknown diterpenoids from this plant for the first time. The chemical structures of the purifiedcompounds were elucidated using spectroscopic analyses including EI-MS, 1H and 13C NMR andby compariso...
متن کاملEsters of Quinoxaline 1,4-di-N-oxide with Cytotoxic Activity on Tumor Cell Lines Based on NCI-60 Panel
Quinoxalines display diverse and interesting pharmacological activities as antibacterial, antiviral, antiparasitic and anticancer agents. Particularly, their 1,4-di-N-oxides derivatives have proved to be cytotoxic agents that are active under hypoxic conditions as that of solid tumours. A new series of quinoxaline 1,4-di-N-oxides substitutes at 7-position with esters group were synthetized and ...
متن کامل